human tead1 vector Search Results


pact2 clontech vector  (TaKaRa)
96
TaKaRa pact2 clontech vector
Pact2 Clontech Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tead1 vector  (Addgene inc)
93
Addgene inc tead1 vector
G-4 (10 μM) disturbed YAP-TEAD interaction. a G-4 treatment disturbed the <t>YAP-TEAD1</t> interaction in the nucleus of HCT15/Tax cells. The YAP-TEAD1 interaction was probed in cells 4 h after G-4 treatment and in untreated cells using co-IP. b ChIP analysis of YAP interaction with the Cyr 61 and COX-2 promoter in HCT15/Tax cells. YAP was examined in cells 4 h after G-4 treatment and in untreated cells. ** P < 0.01 compared with vehicle group. c Cyr 61 and CTGF luciferase reporter activity was measured after treatment with G-4 for 48 h. The fold changes in luciferase activity were calculated by normalizing untreated cells with G-4-treated cells. Data are representative of at least three independent experiments. Error bars represent SD. ** P < 0.01 compared with control cells
Tead1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tead1 vector - by Bioz Stars, 2026-04
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plasmid vector pcmx gal4 tead1  (Addgene inc)
92
Addgene inc plasmid vector pcmx gal4 tead1
G-4 (10 μM) disturbed YAP-TEAD interaction. a G-4 treatment disturbed the <t>YAP-TEAD1</t> interaction in the nucleus of HCT15/Tax cells. The YAP-TEAD1 interaction was probed in cells 4 h after G-4 treatment and in untreated cells using co-IP. b ChIP analysis of YAP interaction with the Cyr 61 and COX-2 promoter in HCT15/Tax cells. YAP was examined in cells 4 h after G-4 treatment and in untreated cells. ** P < 0.01 compared with vehicle group. c Cyr 61 and CTGF luciferase reporter activity was measured after treatment with G-4 for 48 h. The fold changes in luciferase activity were calculated by normalizing untreated cells with G-4-treated cells. Data are representative of at least three independent experiments. Error bars represent SD. ** P < 0.01 compared with control cells
Plasmid Vector Pcmx Gal4 Tead1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yap, cox-2, lats1 shrna  (Addgene inc)
90
Addgene inc yap, cox-2, lats1 shrna
G-4 (10 μM) disturbed YAP-TEAD interaction. a G-4 treatment disturbed the <t>YAP-TEAD1</t> interaction in the nucleus of HCT15/Tax cells. The YAP-TEAD1 interaction was probed in cells 4 h after G-4 treatment and in untreated cells using co-IP. b ChIP analysis of YAP interaction with the Cyr 61 and COX-2 promoter in HCT15/Tax cells. YAP was examined in cells 4 h after G-4 treatment and in untreated cells. ** P < 0.01 compared with vehicle group. c Cyr 61 and CTGF luciferase reporter activity was measured after treatment with G-4 for 48 h. The fold changes in luciferase activity were calculated by normalizing untreated cells with G-4-treated cells. Data are representative of at least three independent experiments. Error bars represent SD. ** P < 0.01 compared with control cells
Yap, Cox 2, Lats1 Shrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yap, cox-2, lats1 shrna - by Bioz Stars, 2026-04
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human tead1  (OriGene)
93
OriGene human tead1
G-4 (10 μM) disturbed YAP-TEAD interaction. a G-4 treatment disturbed the <t>YAP-TEAD1</t> interaction in the nucleus of HCT15/Tax cells. The YAP-TEAD1 interaction was probed in cells 4 h after G-4 treatment and in untreated cells using co-IP. b ChIP analysis of YAP interaction with the Cyr 61 and COX-2 promoter in HCT15/Tax cells. YAP was examined in cells 4 h after G-4 treatment and in untreated cells. ** P < 0.01 compared with vehicle group. c Cyr 61 and CTGF luciferase reporter activity was measured after treatment with G-4 for 48 h. The fold changes in luciferase activity were calculated by normalizing untreated cells with G-4-treated cells. Data are representative of at least three independent experiments. Error bars represent SD. ** P < 0.01 compared with control cells
Human Tead1, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human tead1 - by Bioz Stars, 2026-04
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dna plasmids that encode wild type human yap  (Addgene inc)
90
Addgene inc dna plasmids that encode wild type human yap
G-4 (10 μM) disturbed YAP-TEAD interaction. a G-4 treatment disturbed the <t>YAP-TEAD1</t> interaction in the nucleus of HCT15/Tax cells. The YAP-TEAD1 interaction was probed in cells 4 h after G-4 treatment and in untreated cells using co-IP. b ChIP analysis of YAP interaction with the Cyr 61 and COX-2 promoter in HCT15/Tax cells. YAP was examined in cells 4 h after G-4 treatment and in untreated cells. ** P < 0.01 compared with vehicle group. c Cyr 61 and CTGF luciferase reporter activity was measured after treatment with G-4 for 48 h. The fold changes in luciferase activity were calculated by normalizing untreated cells with G-4-treated cells. Data are representative of at least three independent experiments. Error bars represent SD. ** P < 0.01 compared with control cells
Dna Plasmids That Encode Wild Type Human Yap, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna plasmids that encode wild type human yap - by Bioz Stars, 2026-04
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lats1 shrna  (Addgene inc)
96
Addgene inc lats1 shrna
G-4 (10 μM) disturbed YAP-TEAD interaction. a G-4 treatment disturbed the <t>YAP-TEAD1</t> interaction in the nucleus of HCT15/Tax cells. The YAP-TEAD1 interaction was probed in cells 4 h after G-4 treatment and in untreated cells using co-IP. b ChIP analysis of YAP interaction with the Cyr 61 and COX-2 promoter in HCT15/Tax cells. YAP was examined in cells 4 h after G-4 treatment and in untreated cells. ** P < 0.01 compared with vehicle group. c Cyr 61 and CTGF luciferase reporter activity was measured after treatment with G-4 for 48 h. The fold changes in luciferase activity were calculated by normalizing untreated cells with G-4-treated cells. Data are representative of at least three independent experiments. Error bars represent SD. ** P < 0.01 compared with control cells
Lats1 Shrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lats1 shrna - by Bioz Stars, 2026-04
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megaman human trinscriptome library  (Agilent technologies)
90
Agilent technologies megaman human trinscriptome library
G-4 (10 μM) disturbed YAP-TEAD interaction. a G-4 treatment disturbed the <t>YAP-TEAD1</t> interaction in the nucleus of HCT15/Tax cells. The YAP-TEAD1 interaction was probed in cells 4 h after G-4 treatment and in untreated cells using co-IP. b ChIP analysis of YAP interaction with the Cyr 61 and COX-2 promoter in HCT15/Tax cells. YAP was examined in cells 4 h after G-4 treatment and in untreated cells. ** P < 0.01 compared with vehicle group. c Cyr 61 and CTGF luciferase reporter activity was measured after treatment with G-4 for 48 h. The fold changes in luciferase activity were calculated by normalizing untreated cells with G-4-treated cells. Data are representative of at least three independent experiments. Error bars represent SD. ** P < 0.01 compared with control cells
Megaman Human Trinscriptome Library, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plko 1 neo vector  (Addgene inc)
94
Addgene inc plko 1 neo vector
G-4 (10 μM) disturbed YAP-TEAD interaction. a G-4 treatment disturbed the <t>YAP-TEAD1</t> interaction in the nucleus of HCT15/Tax cells. The YAP-TEAD1 interaction was probed in cells 4 h after G-4 treatment and in untreated cells using co-IP. b ChIP analysis of YAP interaction with the Cyr 61 and COX-2 promoter in HCT15/Tax cells. YAP was examined in cells 4 h after G-4 treatment and in untreated cells. ** P < 0.01 compared with vehicle group. c Cyr 61 and CTGF luciferase reporter activity was measured after treatment with G-4 for 48 h. The fold changes in luciferase activity were calculated by normalizing untreated cells with G-4-treated cells. Data are representative of at least three independent experiments. Error bars represent SD. ** P < 0.01 compared with control cells
Plko 1 Neo Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sapiens ppgs 3ha tead1  (Addgene inc)
92
Addgene inc sapiens ppgs 3ha tead1
Figure 2. <t>Tead1</t> mediates the oncogenic transcriptional program of YAP-TFE3. (A) Knockdown of Tead1 in NIH 3T3 YAP-TFE3 cells. (B) Poly-HEMA analysis in NIH 3T3 YAP-TFE3 cells with Tead1 knock-down. Spheroid formation on poly-HEMA is shown below. (C) Soft agar assay in NIH 3T3 YAP- TFE3 cells with two Tead1 shRNA (shTead#1 and shTead#2) with colonies shown below. (D) Co-immunoprecipitation experiment in NIH 3T3 cells stably expressing Flag-YAP-TFE3 or Flag-YAP-TFE3 S94A. <t>3HA-TEAD1</t> is transiently transfected. * indicates MW of Flag-YAP-TFE3. (E) Poly-HEMA assay in NIH 3T3 cells expressing same constructs present in E. Spheroid formation on poly-HEMA is shown below. (F) Soft agar assay in NIH 3T3 cells expressing YAP-TFE3 (YT), YT S94A, YAP, or YAPS94A. (G) Quantitative RT-PCR showing reduced Ccn2 expression in NIH 3T3 YAP-TFE3 (YT) S94A cells. Figure 2 continued on next page
Sapiens Ppgs 3ha Tead1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β2-chimaerin lentiviral shrna vectors  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare β2-chimaerin lentiviral shrna vectors
Figure 2. <t>Tead1</t> mediates the oncogenic transcriptional program of YAP-TFE3. (A) Knockdown of Tead1 in NIH 3T3 YAP-TFE3 cells. (B) Poly-HEMA analysis in NIH 3T3 YAP-TFE3 cells with Tead1 knock-down. Spheroid formation on poly-HEMA is shown below. (C) Soft agar assay in NIH 3T3 YAP- TFE3 cells with two Tead1 shRNA (shTead#1 and shTead#2) with colonies shown below. (D) Co-immunoprecipitation experiment in NIH 3T3 cells stably expressing Flag-YAP-TFE3 or Flag-YAP-TFE3 S94A. <t>3HA-TEAD1</t> is transiently transfected. * indicates MW of Flag-YAP-TFE3. (E) Poly-HEMA assay in NIH 3T3 cells expressing same constructs present in E. Spheroid formation on poly-HEMA is shown below. (F) Soft agar assay in NIH 3T3 cells expressing YAP-TFE3 (YT), YT S94A, YAP, or YAPS94A. (G) Quantitative RT-PCR showing reduced Ccn2 expression in NIH 3T3 YAP-TFE3 (YT) S94A cells. Figure 2 continued on next page
β2 Chimaerin Lentiviral Shrna Vectors, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgl3-snhg16-pro  (Ribobio co)
90
Ribobio co pgl3-snhg16-pro
Figure 2. <t>Tead1</t> mediates the oncogenic transcriptional program of YAP-TFE3. (A) Knockdown of Tead1 in NIH 3T3 YAP-TFE3 cells. (B) Poly-HEMA analysis in NIH 3T3 YAP-TFE3 cells with Tead1 knock-down. Spheroid formation on poly-HEMA is shown below. (C) Soft agar assay in NIH 3T3 YAP- TFE3 cells with two Tead1 shRNA (shTead#1 and shTead#2) with colonies shown below. (D) Co-immunoprecipitation experiment in NIH 3T3 cells stably expressing Flag-YAP-TFE3 or Flag-YAP-TFE3 S94A. <t>3HA-TEAD1</t> is transiently transfected. * indicates MW of Flag-YAP-TFE3. (E) Poly-HEMA assay in NIH 3T3 cells expressing same constructs present in E. Spheroid formation on poly-HEMA is shown below. (F) Soft agar assay in NIH 3T3 cells expressing YAP-TFE3 (YT), YT S94A, YAP, or YAPS94A. (G) Quantitative RT-PCR showing reduced Ccn2 expression in NIH 3T3 YAP-TFE3 (YT) S94A cells. Figure 2 continued on next page
Pgl3 Snhg16 Pro, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


G-4 (10 μM) disturbed YAP-TEAD interaction. a G-4 treatment disturbed the YAP-TEAD1 interaction in the nucleus of HCT15/Tax cells. The YAP-TEAD1 interaction was probed in cells 4 h after G-4 treatment and in untreated cells using co-IP. b ChIP analysis of YAP interaction with the Cyr 61 and COX-2 promoter in HCT15/Tax cells. YAP was examined in cells 4 h after G-4 treatment and in untreated cells. ** P < 0.01 compared with vehicle group. c Cyr 61 and CTGF luciferase reporter activity was measured after treatment with G-4 for 48 h. The fold changes in luciferase activity were calculated by normalizing untreated cells with G-4-treated cells. Data are representative of at least three independent experiments. Error bars represent SD. ** P < 0.01 compared with control cells

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: YAP transcriptionally regulates COX-2 expression and GCCSysm-4 (G-4), a dual YAP/COX-2 inhibitor, overcomes drug resistance in colorectal cancer

doi: 10.1186/s13046-017-0612-3

Figure Lengend Snippet: G-4 (10 μM) disturbed YAP-TEAD interaction. a G-4 treatment disturbed the YAP-TEAD1 interaction in the nucleus of HCT15/Tax cells. The YAP-TEAD1 interaction was probed in cells 4 h after G-4 treatment and in untreated cells using co-IP. b ChIP analysis of YAP interaction with the Cyr 61 and COX-2 promoter in HCT15/Tax cells. YAP was examined in cells 4 h after G-4 treatment and in untreated cells. ** P < 0.01 compared with vehicle group. c Cyr 61 and CTGF luciferase reporter activity was measured after treatment with G-4 for 48 h. The fold changes in luciferase activity were calculated by normalizing untreated cells with G-4-treated cells. Data are representative of at least three independent experiments. Error bars represent SD. ** P < 0.01 compared with control cells

Article Snippet: DNA plasmids that encode wild type human YAP (hYAP, CMV2-YAP) and TEAD1 vector (pRK5-Myc-TEAD 1) and YAP, COX-2, LATS1 shRNA were obtained from Addgene (USA).

Techniques: Co-Immunoprecipitation Assay, Luciferase, Activity Assay, Control

Figure 2. Tead1 mediates the oncogenic transcriptional program of YAP-TFE3. (A) Knockdown of Tead1 in NIH 3T3 YAP-TFE3 cells. (B) Poly-HEMA analysis in NIH 3T3 YAP-TFE3 cells with Tead1 knock-down. Spheroid formation on poly-HEMA is shown below. (C) Soft agar assay in NIH 3T3 YAP- TFE3 cells with two Tead1 shRNA (shTead#1 and shTead#2) with colonies shown below. (D) Co-immunoprecipitation experiment in NIH 3T3 cells stably expressing Flag-YAP-TFE3 or Flag-YAP-TFE3 S94A. 3HA-TEAD1 is transiently transfected. * indicates MW of Flag-YAP-TFE3. (E) Poly-HEMA assay in NIH 3T3 cells expressing same constructs present in E. Spheroid formation on poly-HEMA is shown below. (F) Soft agar assay in NIH 3T3 cells expressing YAP-TFE3 (YT), YT S94A, YAP, or YAPS94A. (G) Quantitative RT-PCR showing reduced Ccn2 expression in NIH 3T3 YAP-TFE3 (YT) S94A cells. Figure 2 continued on next page

Journal: eLife

Article Title: TAZ-CAMTA1 and YAP-TFE3 alter the TAZ/YAP transcriptome by recruiting the ATAC histone acetyltransferase complex

doi: 10.7554/elife.62857

Figure Lengend Snippet: Figure 2. Tead1 mediates the oncogenic transcriptional program of YAP-TFE3. (A) Knockdown of Tead1 in NIH 3T3 YAP-TFE3 cells. (B) Poly-HEMA analysis in NIH 3T3 YAP-TFE3 cells with Tead1 knock-down. Spheroid formation on poly-HEMA is shown below. (C) Soft agar assay in NIH 3T3 YAP- TFE3 cells with two Tead1 shRNA (shTead#1 and shTead#2) with colonies shown below. (D) Co-immunoprecipitation experiment in NIH 3T3 cells stably expressing Flag-YAP-TFE3 or Flag-YAP-TFE3 S94A. 3HA-TEAD1 is transiently transfected. * indicates MW of Flag-YAP-TFE3. (E) Poly-HEMA assay in NIH 3T3 cells expressing same constructs present in E. Spheroid formation on poly-HEMA is shown below. (F) Soft agar assay in NIH 3T3 cells expressing YAP-TFE3 (YT), YT S94A, YAP, or YAPS94A. (G) Quantitative RT-PCR showing reduced Ccn2 expression in NIH 3T3 YAP-TFE3 (YT) S94A cells. Figure 2 continued on next page

Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Mus musculus) NIH 3T3 cells ATCC CRL-1658; RRID:CVCL_0594 Mycoplasma negative Cell line (Homo sapiens) SW872 cells ATCC HTB92; RRID:CVCL_1730 Mycoplasma negative Transfected construct (H. sapiens) pPGS-3HA-TEAD1 Dr. Kunliang Guan (Addgene) #33055; RRID:Addgene_33055 Transfected construct (H. sapiens) 8xGTIIC-luciferase Dr. Stefano Piccolo (Addgene) #34615; RRID:Addgene_34615 Transfected construct (H. sapiens) pRP-CMV-3xMyc-KAT14 Vector builder Commercial assay or kit SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling #9003 Antibody Anti-DYKDDDDK antibody Cell Signaling #14793; RRID:AB_2572291 ChIP grade antibody Merritt, Garcia, et al. eLife 2021;10:e62857.

Techniques: Knockdown, Soft Agar Assay, shRNA, Immunoprecipitation, Stable Transfection, Expressing, Transfection, Construct, Quantitative RT-PCR